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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperChrom AR rProtein A/G Agarose 4FF Agarose is an affinity chromatography medium for antibody purification, separated by specific interaction between antigens and antibodies, mainly used for the purification of IgG in culture media, serum, ascites or hybridoma cell culture supernatant, or the purification of recombinant proteins containing Fc fragments.
The HyperChrom AR rProtein A FF Agarose and HyperChrom Protein G 4FF Agarose in this product are covalently attached to 4% high crosslinked, high flow rate agarose and mixed in a 1:1 ratio. Each milliliter of HyperChrom AR rProtein A/G Agarose 4FF Agoros can bind approximately 20 mg of human IgG.
HyperChrom AR rProtein A/G Agarose 4FF Agarose chromatography media parameters
Name
Description
Chromatography media type
Affinity chromatography media
Ligation
rProtein A/G (E. coli recombinant expression, 1:1).
Scaffolding
Highly cross-linked agarose
Average particle size
90 μm
Dynamic load
~20 mg human IgG/mL chromatography medium
Flow rates are recommended
50-400 cm/h
Withstand pressure
0.3 MPa
Use temperature
4-30℃
pH stability
3-9
Store the components at 4℃ for 5 years.
