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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperChrom Butyl 4FF Agarose is a hydrophobic interaction chromatography medium that takes advantage of differences in the hydrophobic properties and the amount of different molecules under specific conditions. This series of chromatography media can be well used for the isolation and purification of a variety of biomolecules, such as recombinant proteins, antibodies, viral vaccines, etc.
HyperChrom Butyl 4FF Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base frame with excellent rigidity, so high process flow rates and improved process efficiency at low backpressure. (2) Hydrophilic base frame to minimize the influence on the hydrophobic role of the ligand. (3) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.
HyperChrom Butyl 4FF Agarose chromatography media parameters
Name
Description
Chromatography media type
Hydrophobic interactions
Ligation
R-O-CH2-CH(OH)-CH2-O-(CH2)3-CH3
Scaffolding
Highly cross-linked agarose
Average particle size
90 μm
Ligand density
40 μmol Butyl group/mL chromatography medium
Flow rates are recommended
150-250 cm/h
Maximum flow rate
400 cm/h
Withstand pressure
0.3 MPa
Use temperature
4-30℃
pH stability*
2-14
Solvent resistant
Common aqueous solution, 3 M ammonium sulfate, 30% isopropanol**, 75% ethanol**, 1 M NaOH, 6 M guanidine hydrochloride
Intolerant to solvents
oxidant
* After 7 days of storage of chromatography medium at 40oC and pH 2-14 environment, its physicochemical properties and functions did not change significantly.
**30% and 75% are v/v, volume ratio.
Store the components at 4℃ for 5 years.