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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperChrom CM FF Agarose is a weak cation exchange chromatography medium that separates molecules using differences in charge properties and amounts under specific conditions. It was found that when sodium hydroxide solution was continuously added dropwise to the chromatography medium, measuring the pH of the supernatant found that it could maintain a fairly wide range of pH stability. It is precisely because of this property that HyperChrom CM FF Agarose chromatography media exhibits an unexpected separation and purification effect. The chromatography medium can be well used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc.
HyperChrom CM FF Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base frame with excellent rigidity, so it can achieve high process flow rates and improve process efficiency at low back pressure. (2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.
HyperChrom CM FF Agarose chromatography media parameters
Name
Description
Chromatography media type
Weak cation exchange
Ligation
-O-CH2COO-
Scaffolding
Highly cross-linked agarose
Average particle size
90 μm
Ion load
0.09-0.13 mmol H+ /mL chromatography medium
Dynamic load
> 50 mg lysozyme/mL chromatography medium*
Flow rates are recommended
300-600 cm/h
Maximum flow rate
700 cm/h
Withstand pressure
0.3 MPa
Use temperature
4-30℃
pH stability **
2-14
Solvent resistant
Common aqueous solution, 30% isopropanol***, 75% ethanol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea
Intolerant to solvents
Oxidant, cationic detergent
* Dynamic load measurement conditions: column loading height: 10cm, test flow rate 300 cm/h; Test buffer: 0.1 M citrate solution, pH 3.0; test sample: 4 mg/mL lysozyme sample, when the penetration of lysozyme reaches 10%, the lysozyme sample volume per unit volume (mL) (mg).
**After 7 days in the environment of chromatography at 40°C and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly.
***30% and 75% are v/v, volume ratio.
Store the components at 4℃ for 5 years.
