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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperChrom Phenyl 6FF (HS) Agoros is a class of hydrophobic interaction chromatography media that takes advantage of differences in the hydrophobic properties and the amount of different molecules under specific conditions. This chromatography medium is mainly used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, viral vaccines, etc.
The HyperChrom Phenyl 6FF (HS) Agoros series offers excellent scale-up performance: (1) Highly crosslinked agarose base with excellent rigidity, resulting in high process flow rates and improved process efficiency at low backpressure. (2) Hydrophilic base frame to minimize the influence on the hydrophobic role of the ligand. (3) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide. (4) Fine particle size design to improve resolution.
HyperChrom Phenyl HP Agarose chromatography media parameters
Name
Description
Chromatography media type
Hydrophobic interactions
Ligation
R-O-CH2-CH(OH)-CH2 -O-C6H5
Scaffolding
Highly cross-linked agarose
Average particle size
34 μm
Ligand density
25 μmol phenyl group/mL chromatography medium
Dynamic load
>45 mg α-chymotrypsinogen/mL chromatography medium*
Flow rates are recommended
90-150 cm/h
Maximum flow rate
200 cm/h
Withstand pressure
0.3 MPa
Use temperature
4-30℃
pH stability**
2-14
Solvent resistant
Common aqueous solution, 3 M ammonium sulfate, 30% isopropanol***, 75% ethanol***, 10% ethylene glycol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea
Intolerant to solvents
Oxidant
*Dynamic load measurement conditions: column height: 10 cm, test flow rate 150 cm/h, test buffer: 0.1 M potassium phosphate, 2 M ammonium sulfate solution, pH 7.0, test sample: 2mg/m L of α-chymotrypsinogen sample, when the penetration of α-chymotrypsinogen reaches 10%, the amount of α-chymotrypsinogen per unit volume (mL) (mg).
**After 7 days in the environment of chromatography at 40oC and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly.
*** 30%, 75% and 10% are v/v, volume ratio.
Store the components at 4℃ for 5 years.
