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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperChrom SP FF Agarose is a strong cation exchange chromatography medium that separates molecules using differences in charge properties and amounts under specific conditions. The chromatography medium can be well used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc.
HyperChrom SP FF Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base frame with excellent rigidity, so it can achieve high process flow rates and improve process efficiency at low back pressure. (2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.
HyperChrom SP FF Agarose chromatography media parameters
Name
Description
Chromatography media type
Strong cation exchange
Ligation
- (CH2)3SO3- (sulfopropyl).
Scaffolding
Highly cross-linked agarose
Average particle size
90 μm
Ion load
0.18-0.25 mmoll H+/mL chromatography medium
Dynamic load
>50 mg β-lactoglobulin/mL chromatography medium*
Flow rates are recommended
300-600 cm/h
Maximum flow rate
700 cm/h
Withstand pressure
0.3 MPa
Use temperature
4-30℃
pH stability **
2-14
Solvent resistant
Common aqueous solution, 30% isopropanol***, 75% ethanol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea
Intolerant to solvents
Oxidant, cation detergent
* Dynamic load measurement conditions: column loading height: 10cm, test flow rate 300 cm/h; Test buffer: 0.1 M Citrate solution, pH 3.0; test sample: 4 mg/ml β-lactoglobulin sample, when the penetration of β-lactoglobulin reaches 10%, the amount of β-lactoglobulin per unit volume of media (mL) (mg).
**After 7 days in the environment of chromatography at 40°C and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly.
***30% and 75% are v/v, volume ratio.
Store the components at 4℃ for 5 years.