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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperScribeTM T7 High Yield RNA Synthesis Kit performs in vitro RNA transcription experiment in a relatively short time using T7 RNA polymerase. The addition of N1-Methylpseudo-UTP(m1Ψ) (Cat. No. B8049) can reduce host cell immune response. The kit is designed for high yields of RNA transcripts, highly specific radiolabeled RNA probes and to obtain capped, dye-labeled or biotinylated RNA incorporated modified nucleotides. RNA synthesized using this kit covers a wide range of applications, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.
The kit contains sufficient reagents to carry out 25/50/100 reactions, 20 μL each time. Up to 180 μg of RNA can be generated with 1 μg of control template per standard reaction.
Components
25 rxns
50 rxns
100 rxns
T7 RNA Polymerase Mix
50 μL
100 μL
200 μL
10 × Reaction Buffer
ATP (100 mM)
25 μL
GTP (100 mM)
N1-Methylpseudo-UTP (100 mM)
CTP (100 mM)
Control Template (0.5 μg/μL)
5 μL
10 μL
20 μL
RNase-free H2O
0.5 mL
1 mL
2 x 1 mL
Store the components at -20°C.