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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperScript™ III Reverse Transcriptase is a third-generation reverse transcriptase based on M-MLV Reverse Transcriptase that has been genetically engineered. Compared to wild-type, HyperScript™ III Reverse Transcriptase generates a reduced RNase H activity and improves thermal stability and fidelity. In addition, it has characteristics of higher cDNA synthesis yield and longer length, higher reverse transcription efficiency for high-GC content RNA. HyperScript™ III Reverse Transcriptase has an enhanced affinity for templates and is suitable for reverse transcription of small amounts of template and low-copy genes, you can use PCR to amplify cDNA up to 12.3 kb.
HyperScript™ III First-Strand cDNA Synthesis SuperMix (with gDNA wiper) is a kit based on HyperScript™ III Reverse Transcriptase, which contains all components except primers and templates required for the reaction. The kit supplies Random Primers and Oligo (dT)23VN primer. The inclusion of the 4× gDNA wiper in the product is intended to remove potential genomic DNA contamination before reverse transcription. Random Primers, Oligo (dT)23VN or gene-specific primers can be chosen as reverse transcription primers according to your needs, and the synthesized first-strand cDNA products can be used in subsequent PCR amplification, qPCR reactions, and other experiments.
Store the components at -20℃ for 2 years.