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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
LDH Cytotoxicity Assay Kit (Fluorometric) is a kit that assesses cytotoxicity by measuring the activity of lactate dehydrogenase (LDH) released into culture media. LDH is a stable enzyme found in all cells and is released from the cell into the medium when cells are damaged or apoptosis. LDH catalyzes the conversion of lactate to pyruvate, and this catalytic process reduces NAD+ to NADH. The specific substrates provided in this kit can react with the resulting NADH to produce a red fluorescent product. The fluorescence intensity was positively correlated with LDH activity. Therefore, by measuring the fluorescence intensity, it is possible to indirectly assess the degree of cell damage or death.
Compared with the traditional 51Cr cytotoxicity test, this kit contains no radioactive substances, which is simpler and safer to use. Moreover, the detection effect of this kit is consistent with the 51Cr test, and the results are reliable. The main difference between this kit and the LDH Cytotoxicity Assay Kit (K2228) is the detection method, this kit detects fluorescence, and K2228 detects absorbance, which can be selected according to specific needs.
Figure 1: LDH release as measured by this kit
K2267-200T
K2267-1000T
Substrate Mix
1 vial
5 x 1 vial
Reporter Mix
12 mL
5 x 12 mL
Lysis Buffer (10X)
2.5 mL
Stop Solution
60 mL
LDH Positive Control
6 μL
30 μL
Store the kit at -20°C, stable for 1 year. Substrate Mix should be stored away from light.