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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The Malachite Green Phosphate Assay Kit is a kit for the detection of phosphate concentration. The principle is that under acidic conditions, malachite green, molybdate and free inorganic orthophosphate can form a green phosphomolybdate complex, and the absorbance of this green phosphomolybdate complex at 650 nm is proportional to the concentration of free inorganic phosphate in solution.
This kit can quickly, conveniently and sensitively determine free inorganic phosphate in solution, so it is often used for the determination of phosphate concentration in solution and the ATPase activity. However, this kit is only suitable for the determination of free phosphate in solution. For the bound phosphate, such as binding to lipids or proteins, certain methods must be used for release before detection. The kit has a good linear relationship in the range of 0-10 nmol during detection.
Figure 1: Reference standard curve of this kit
Store the kit at 4°C, stable for 1 year. Malachite Green Reagent B should be stored away from light.