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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
MTT Cell Proliferation Assay Kit is a widely used cell proliferation and toxicity assay kit. MTT, a tetrazolium salt, can be reduced by succinate dehydrogenase in the mitochondria of living cells to the water-insoluble purple formazan crystals. The amount of formazan can be quantified by measuring the absorbance at 570 nm upon solubilization and is proportional to the number of living cells.
This kit provides a special formazan solvent, which does not need to remove the original medium and can be directly added to dissolve the formazan. At the same time, this kit is easy to use, highly sensitive and has a good linear range.
Figure 1: Figure 1: Reference standard curve of this kit
Components
K2249-500T
K2249-2500T
MTT
25 mg
125 mg
MTT Solvent
5 mL
25 mL
Formazan Solvent
50 mL
250 mL
Store the kit at -20°C, stable for 1 year. MTT should be stored away from light and moisture.