require.config({ deps: [ 'jquery', 'mage/translate', 'jquery/jquery-storageapi' ], callback: function ($) { 'use strict'; var dependencies = [], versionObj; $.initNamespaceStorage('mage-translation-storage'); $.initNamespaceStorage('mage-translation-file-version'); versionObj = $.localStorage.get('mage-translation-file-version'); if (versionObj.version !== '2945a803802eff1885867c4f1ead83ea9223774a') { dependencies.push( 'text!js-translation.json' ); } require.config({ deps: dependencies, callback: function (string) { if (typeof string === 'string') { $.mage.translate.add(JSON.parse(string)); $.localStorage.set('mage-translation-storage', string); $.localStorage.set( 'mage-translation-file-version', { version: '2945a803802eff1885867c4f1ead83ea9223774a' } ); } else { $.mage.translate.add($.localStorage.get('mage-translation-storage')); } } }); } }); { "*": { "mage/cookies": { "expires": null, "path": "/", "domain": ".www.apexbt.com", "secure": false, "lifetime": "3600" } } }<div class="message global noscript"><div class="content"><p><strong>JavaScript seems to be disabled in your browser.</strong> <span>For the best experience on our site, be sure to turn on Javascript in your browser.</span></p></div></div>

Home
Nisoldipine

Nisoldipine

Catalog No.

B1989

calcium channel blocker

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Grouped product items
Size	Price	Stock	Qty
10mM (in 1mL DMSO)	$55.00	In stock
500mg	$64.00	In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background
Product Citation
Chemical Properties
Protocol
Quality Control

Background

Nisoldipine is a calcium channel blocker belonging to the dihydropyridines class, specific for L-type Cav1.2 with IC50 of 10 nM.

Product Citation

1. Qianyue Chen, Jiashuo Zheng, et al. "Nitrosonisoldipine is a selective inhibitor of inflammatory caspases and protects against pyroptosis and related septic shock." Eur J Immunol. 2021 May;51(5):1234-1245. PMID:33454984

Chemical Properties

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	388.41
Cas No.	63675-72-9
Formula	C₂₀H₂₄N₂O₆
Solubility	insoluble in H2O; ≥10.62 mg/mL in EtOH; ≥11.55 mg/mL in DMSO
Chemical Name	3-O-methyl 5-O-(2-methylpropyl) 2,6-dimethyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate
SDF	Download SDF
Canonical SMILES	CC1=C(C(C(=C(N1)C)C(=O)OCC(C)C)C2=CC=CC=C2[N+](=O)[O-])C(=O)OC
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Kinase experiment [1]:
Binding experiments of electrophysiology	CHO cells expressing the subunit of the voltage-dependent L-type Ca2+ channel were cultrured in medium without serum in the presence of different concentrations of Nisoldipine. Then Ca2+ channel current elicited from a holding potential of -100 mV or -50 mV was recorded at room temperature with the whole-cell configuration of the patch-clamp method using the List EPC-7 patch-clamp amplifer and pClamp software. The IC50 values were calculated.
Cell experiment [2]:
Cell lines	Guinea-pig ventricular myocytes
Preparation method	The solubility of this compound in DMSO is > 11.6 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.
Reacting condition	10 ~ 100 μM; 8 ~ 10 mins
Applications	In guinea-pig ventricular myocytes, Nisoldipine inhibited rapidly activating delayed-rectifier K+ current (I(Kr) ) with an IC50 value of 23 μM, as well as slowly activating delayed-rectifier K+ current (I(Ks) ) with an IC50 value of 40 μM. It was estimated that Nisoldipine is approximately 30 times more selective for L-type Ca2+ channels than for delayed-rectifier K+ channels.
Animal experiment [3]:
Animal models	A dog model of coronary ischemia-reperfusion
Dosage form	6.6 μg/kg/min; i.v.
Applications	Compared with the control group, the Nisoldipine treatment group showed similar hemodynamic measurements. However, mass of necrosis and mass at risk was obviously lower in the Nisoldipine group. In a dog model of coronary ischemia-reperfusion, Nisoldipine reduced the infarct size.
Other notes	Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References: [1]. Morel N, Buryi V, Feron O, Gomez JP, Christen MO, Godfraind T. The action of calcium channel blockers on recombinant L-type calcium channel alpha1-subunits. Br J Pharmacol. 1998 Nov;125(5):1005-12. [2]. Missan S1, Zhabyeyev P, Dyachok O, Jones SE, McDonald TF. Block of cardiac delayed-rectifier and inward-rectifier K+ currents by nisoldipine. Br J Pharmacol. 2003 Nov;140(5):863-70. [3]. Hammerman H, Moscovitz M, Hir J. Beneficial effect of nisoldipine in repeated coronary reperfusion. Coron Artery Dis. 1997 Feb;8(2):97-100.

Quality Control

Quality Control & MSDS

View current batch:

Purity = 99.25%

Chemical structure

Nisoldipine

Related Biological Data

Nisoldipine

Related Biological Data

Nisoldipine