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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell lines
Peripheral blood lymphocytes (PBL) or peripheral CD4+ and CD8+ T cell subsets
Preparation method
The solubility of this compound in DMSO is > 15.4 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.
Reacting condition
10-3 ~ 10-12 M; 72, 96 and 120 hrs
Applications
Prednisone arrested PBL in the G1 phase of cell cycle, and inhibited IL-2 receptor (IL-2R) expression as well as IL-2 secretion. In phytohaemaglutinin (PHA)-activated human PBL, Prednisone significantly increased apoptosis. Moreover, Prednisone exhibited stronger apoptotic effect on CD8+ T lymphocytes than on CD4+ T lymphocytes. All these effects were shown in dose- and time-dependent manners.
Animal models
Male Wistar rats
Dosage form
5 mg/kg/d; p.o.; for 90 days
In Wistar rats, Prednisone significantly delayed learning and memory retention by 20%. Compared with the control group, the neuronal degeneration index in the Prednisone treatment group was 2 times higher in the prefrontal cortex, and about 10 times higher in the CA1 hippocampus. In addition, Prednisone substantially increased the number and cytoplasmic transformation of astrocytes, as well as isolectin-B4-labeled microglia cells in the prefrontal cortex.
Other notes
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References:
[1]. Lanza L, Scudeletti M, Puppo F, Bosco O, Peirano L, Filaci G, Fecarotta E, Vidali G, Indiveri F. Prednisone increases apoptosis in in vitro activated human peripheral blood T lymphocytes. Clin Exp Immunol. 1996 Mar;103(3):482-90.
[2]. Ramos-Remus C, González-Castaeda RE, González-Perez O, Luquin S, García-Estrada J. Prednisone induces cognitive dysfunction, neuronal degeneration, and reactive gliosis in rats. J Investig Med. 2002 Nov;50(6):458-64.
