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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. Protein production is greatly halted and degradation is increased when proteins are extracted from cells and tissues in vitro. The best way to increase the yield of intact proteins is to add a cocktail of inhibitors of those enzymes known to be present.
This protease inhibitor cocktail contains components such as Aprotinin, Bestatin, E-64, Leupeptin with a broad specificity for cysteine, serine, acid protease and aminopeptidase. This product does not contain AEBSF, to avoid interfering with mass spectrometry (MS) analysis due to mass spectral peak drift caused by AEBSF. Therefore, protein samples extracted with this product are compatible with mass spectrometry detection. This product is supplied as a ready-to-use solution in DMSO. In addition, an additional EDTA is available for optional use.
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Catalog No.
Product Name
Summary
Targets
CAS Number
Smiles
A (50X in DMSO)
A2574
Aprotinin
Bovine trypsin inhibitors
Proteases|Serine Protease
9087-70-1
A2575
Bestatin
Aminopeptidase inhibitors
Proteases|Aminopeptidase
58970-76-6
CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O
A2576
E-64
Cysteine protease inhibitors
Proteases|Cathepsin
66701-25-5
CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O
A2570
Leupeptin
Serine and cysteine protease inhibitors
103476-89-7
O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H]
B (50X in ddH2O)
EDTA, disodium salt, dihydrate
Metalloproteinase inhibitors
Proteases | Metalloprotease
6381-92-6
Store at -20 °C for one year.
