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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Reactive Oxygen Species Assay Kit is a kit that uses the fluorescent probe DCFH-DA to quantitatively assess reactive oxygen species (ROS) in live cells. DCFH-DA is a cell-permeable probe, which is deacetylated by cellular esterases to a non-fluorescent DCFH. DCFH then is oxidized by ROS into highly fluorescent DCF. The fluorescence intensity of DCF is proportional to the ROS levels. This kit also provides Rosup as a positive control for ROS generation. The concentration of Rosup is 50 mg/mL.
Figure 1: The efficiency of this kit for ROS detection
This kit should be stored at -20°C, stable for 1 year. DCFH-DA should be stored at -20°C away from light, and avoid repeated freeze/thaw cycles.