JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.
Tel: +1-832-696-8203
Email: [email protected]
Worldwide Distributors
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
(S)-Methylisothiourea sulfate is a potent, selective and competitive inhibitor of the inducible nitric oxide synthase (iNOS). Nitric oxide synthases, composed of the constitutive and the inducible isoforms, are a family of enzymes responsible for catalyzing the production of nitric oxide (NO) from L-arginine. The inducible isoform, i.e. iNOS, can be induced in various cells, including macrophages, by a variety of agents such as endotoxin, and thereby produces NO as an immune defense mechanism. Enhanced NO formation has been implied in the pathophysiology of a variety of diseases, such as circulatory shock. Thus, (S)-methylisothiourea sulfate, as a selective iNOS inhibitor, may have considerable value in the therapy of circulatory shock of various etiologies and other pathophysiological conditions associated with induction of iNOS.
Reference:
1. Szabó C, Southan GJ, Thiemermann C. Beneficial effects and improved survival in rodent models of septic shock with S-methylisothiourea sulfate, a potent and selective inhibitor of inducible nitric oxide synthase. Proceedings of the National Academy of Sciences of the United States of America, 1994, 91(26): 12472-12476.
Cell lines
J774.2 macrophages and rat aortic vascular smooth muscle cells
Reaction Conditions
100 nM ~100 μM (S)-methylisothiourea sulfate
Applications
In J774.2 macrophages and rat aortic vascular smooth muscle cells, (S)-methylisothiourea sulfate exhibited more potent inhibitory effects on lipopolysaccharide (LPS)-induced nitrite production than any other known inhibitor of NOS, such as NG-amino-L-arginine.
Animal models
Male Wistar rats, 260 ~ 320 g
Dosage form
0.01 ~ 3 mg/kg
Intravenous administration
S-Methylisothiourea sulfate dose-dependently reversed the hypotension and the vascular hyporeactivity to vasoconstrictor agents caused by endotoxin LPS in anesthetized rats.
Note
The technical data provided above is for reference only.
References: