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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Hygromycin B is a selective antibiotic.
Antibiotics are a type of antimicrobial used in the treatment and prevention of bacterial infection.
Hygromycin B is an antibiotic that is effective on most bacteria, fungi and higher eukaryotes. Hygromycin B has a single binding site within the 30S subunit, which is in consistent with its monophasic effect. The binding site is located in the major groove of the helix with a highly sequence-specific way. Hygromycin B inhibits protein synthesis by inhibiting the translocation step of elongation and causing mistranslation of mRNA in the 70S ribosome [1]. Hygromycin B selectively inhibited activity of the ribosomal ATPase (RbbA) on 70S ribosomes and releases RbbA from 70S ribosomes at physiological concentrations. RbbA enhances the reactivity of A889 and G890 of the 16S rRNA switch helix region. Also, Hygromycin B protects G1494 and A1408 in the decoding region.
References:[1] Brodersen DE, Clemons WM Jr, Carter AP, et al. The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. Cell, 2000, 103 (7): 1143-1154.[2] Ganoza MC, Kiel MC. A ribosomal ATPase is a target for hygromycin B inhibition on Escherichia coli ribosomes. Antimicrob Agents Chemother, 2001, 45 (10): 2813-2819.
Cell lines
Mouse L cells
Reaction Conditions
200 μg/ml hygromycin B for 2 ~ 6 weeks
Applications
Hygromycin B was used for resistance selection in mouse L cells transfected withEscherichia coli genes coding for resistance to the aminocyclitol antibiotics hygromycin B.
Animal models
Four-week-old BALB/c mice susceptible to MHV-A59 infection
Dosage form
0 ~ 10 μmol/kg
Intraperitoneal (i.p.) injection
A single injection of hygromycin B reduced the number of lesions in the livers of MHV-A59-inoculated mice. Higher doses of the drug appeared to be slightly more effective at reducing virus-induced lesions.
Note
The technical data provided above is for reference only.
References:
1. Santerre RF, Allen NE, Hobbs JN Jr, et al. Expression of prokaryotic genes for hygromycin B and G418 resistance as dominant-selection markers in mouse L cells. Gene, 1984, 30(1-3): 147-156.
2. Macintyre G, Curry B, Wong F, et al. Hygromycin B therapy of a murine coronaviral hepatitis. Antimicrobial Agents and Chemotherapy, 1991, 35(10): 2125-2127.