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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell lines
Human glioblastoma multiforme (GBM), rat prostate cancer (PC) and normal mouse 3T3 cells
Reaction Conditions
1 ~ 14 mM sodium ascorbate
Applications
Sodium ascorbate considerably decreased the proliferation and motility of GBM and PC cells. This effect was accompanied by intracellular ROS over-production and necrotic death of tumor cells, apparently resulting from their "autoschizis".
Animal models
Male Wistar rats, 200 ~ 230 g
Dosage form
1 or 2 mg/kg
Injected intravenously at 3rd and 7th day after U87 cell implantation
In U87 tumor-bearing rats, intravenous administration of sodium ascorbate inhibited tumor invasion in vivo and seemed to reduce the size of neoplasia. Concomitantly, blood analyses did not show any signs of hemolysis or biochemical disorders after sodium ascorbate administration. High doses of sodium ascorbate were demonstrated to interfere with the expansion of GBM cells in vitro and in vivo.
Note
The technical data provided above is for reference only.
References:
1. Ryszawy D, Pudełek M, Catapano J, et al. High doses of sodium ascorbate interfere with the expansion of glioblastoma multiforme cells in vitro and in vivo. Life Sciences, 2019, 232: 116657.