JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.
Tel: +1-832-696-8203
Email: [email protected]
Worldwide Distributors
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell lines
HCT116 cells
Reaction Conditions
20 and 40 μmol/L solasodine for 48 h incubation
Applications
Solasodine significantly inhibited the potential of colony spheroid formation strengthened by TGF-β1 in a concentration-dependent manner. Meanwhile, the mRNA and protein levels of stemness markers including CD133, CD44, Nanog, Oct-4 and Sox-2 were down-regulated with increasing concentrations of solasodine.
Animal models
BALB/c/nu/nu nude mice (6 ~ 8 week old, 18 ~ 22 g body weight, half male and half female) xenografted with HCT116 cells
Dosage form
30 and 50 mg/kg
Once daily by intraperitoneal injection for 5 weeks
Tumor xenografts in both low- (30 mg/kg) and high-dose (50 mg/kg) solasodine groups grew more slowly, and the average volume and weight of the finally resected tumors in solasodine-treated groups were remarkably lower. In harvested tumors, stemness and epithelial-mesenchymal transition (EMT) related molecules were both down-regulated. The results of this study implied that solasodine may be a novel therapeutic drug for human colorectal cancer treatment.
Note
The technical data provided above is for reference only.
References:
1. Zhuang YW, Wu CE, Zhou JY, et al. Solasodine reverses stemness and epithelial-mesenchymal transition in human colorectal cancer. Biochemical and Biophysical Research Communications, 2018, 505(2): 485-491.